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HERDIN Record #: PCHRD13013013233869 Submitted: 30 January 2013 Modified: 13 February 2013

Direction detection of DNA of mycobacterium tuberculosis in the liver tissue using polymerase chain reaction (PCR).

D D. Payawal,
M Matsumura,
Y Shiratori,
J D. Sollano Jr,
T Okudira,
R R. Gonzales,
R A. Lopez,
M Omata

Fifteen formalin fixed paraffin embedded liver tissue collected between 1990-1995 were studied. Six (40%) had concomitant ascites of which microscopic analysis revealed predominance of lymphocytes. Miliary and pulmonary tuberculosis were presented in 1(6%) and 6 cases( 40%), respectively. Genomic DNA was extracted and subjected to PCR assay. Oligonucleotides of 123-bp belonging to insertional sequence IS6110 that is repeated and highly specific for Mycobacterium tuberculosis were used as primers. The specificity of PCR was confirmed by Southern blot hybridization using digoxygenin-ddUTP labeled probe. The PCR assay was positive and correctly identified in 50% (7/15) of cases with hepatic tuberculosis. All the 2 cases with classic histopathological features of tuberculosis were also PCR positive and 5 out of 13 cases (40%) with granuloma were positive for Mycobacterium tuberculosis by PCR. Thus, this assay is an efficient alternative method for detecting liver tuberculosis directly, especially when liver biopsy sample is inadequate.

Publication Type
Biennial Report
University of Santo Tomas Research Report 1994-1996
Publication Date
January 1996

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