Bulk extraction of ipil-ipil seed gum was done on coated seeds. The ipil-ipil seeds were ground using a blender. They were then placed in boiling water to disperse the gum. The solution was then filtered. The crude gum was purified by fractional precipitation. The ipil-ipil seed gum is a cream to light brown powder with a slight beany odor. The gum was also subjected to chemical analyses.
The results of the Micronucleus Test showed that the ipil-ipil seed gum is not mutagenic at dosages of 3 mg and 50 mg/kg mouse. At a dosage of 3 mg/kg body weight, the gum reduced by 57.2% the number of micronucleated polychromatic erythrocytes (MN-PCE) induced by the mutagen tetracycline. An increase in the dosage of the gum to 50 mg/kg mouse led to an 81.0% reduction in MN-PCE. Thus the ipil-ipil seed gum is antimutagenic.
Decoating of the ipil-ipil seeds was affected by the use of a cyclone separator. The ipil-ipil seeds were ground using a cutter, sieved through a Tyler mesh, and then placed in the cyclone separator was then used to control separation. The big particles were left inside the cyclone separator while lighter particles were entrained in air and collected in the receiving hopper. (Author)
The study aimed to: 1) identify alternative seed decoating procedure of ipil-ipil; 20 to determine time and cost-efficient isolation procedure; 3) to conduct in vivo mutagenicity and antimutagenicity testing of the ipil-ipil; 4) to produce 1 kg of the ipil-ipil seed gum for pharmaceutical viability testing seed gum.
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