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HERDIN Record #: R07-USC-13072612131551 Submitted: 26 July 2013 Modified: 13 November 2013

Antimicrobial activity of the mature fruit of Diospyros discolor willd. (Mabolo)


This study aimed to determine the antimicrobial effect of Diospyros discolor Willd. (Mabolo) juice on bacteria and fungus which were indicated by the formation of zone inhibitions, a clear, circular zone around the disk containing antimicrobial agent. The bacteria used in the experiment were gram- negative P. aeruginosa and gram- positive S. aureus. For fungus, C. albicans was used. The positive controls used for the experiment setting were Gentamicin and Nystatin for bacteria and fungus, respectively. The chemical constituents of mabolo juice which may be responsible for the antimicrobial effect were identified through test tube phytochemical screening. The result showed that the Mabolo juice (MJ) contains alkaloids, terpenes, reducing sugars and tannins. Among the different compounds studied, alkaloids and tannins may be responsible for the antimicrobial effect since previous studies have shown that these constituents showed antimicrobial activities. MJ100%, MJ50% and MJ10% were the concentrations used in the experiment. Three replicates were made of every trial. For bacteria, observation was done after 24th hour and fungus after 48th hour incubation period. The juices had varying antimicrobial activities against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans except for both the negative and the MJ10% which showed 0% or no antimicrobial activity against the 3 microorganism. MJ100% showed 34.43% MJ50% showed 25.20% and the positive control, showed 100.01% antimicrobial against S. aureus. For P. aeruginosa, MJ100% showed 33.09%, MJ50% showed 27.09% and positive control showed 59.16% and the positive control, Nystatin showed 99.99% antimicrobial. Results showed that the higher the concentration of MJ, the greater the effect, thus, MJ100% was the most effective. Perform an extensive extraction, conduction of phytochemical screening of all the parts of the test plant, and extraction, isolation and purification of the constituents present.

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Pharmacy
Publication Date
March 2011

Objectives

This study was conducted to determine the antimicrobial activity of the mature fruit of Diospyros discolor Willd. (Mabolo) against gram-positive Staphylococcus aureus, gram-negative Pseudomonas aeruginosa and fungus, Candida albicans.
Specifically, the study aimed to:
1. determine the diameter of zone of inhibition (ZOI) exhibited by the juice from the mature fruit of mabolo.
2. determine the percent antimicrobial activity of mabolo juice relative to the positive controls namely, Gentamicin 10mcg sensi disc for bacteria and Nystatin oral suspension (100,000 USP unit per mL) for fungus.
3. identify the microorganism which is most susceptible to the mabolo juice.
4. conduct phytochemical screening on mabolo juice.

LocationLocation CodeAvailable FormatAvailability
University of San Carlos - Josef Baumgartner Learning Resource Center, Science and Technology Section T/M314m Abstract Print Format
1. Aguinaldo, H. A.. "Recent development in the discovery of biologically active natural products" ,
2. Angenot, L. , Quentin-Lerlercq, D. J., Phillipson, D. C.. "Antiamoebic and antiplasmodial activities of alkaloids isolated from strychnos usambarensis" 57, 337-340,
3. Bonjean, K. , De Pauw-Gellet, M. C., Defrense, M. P., Colson, P. , Houssier, C. . "The DNA intercalating alkaloid cryptolepine interferes with topoisomerase II and inhibits primarily DNA synthesis in B16 melamona cells" 37, 1536-1546,
4. Drica, K. , Zhao, X. . "DNA gyrase topoisomerase IV and the 4-quinolones" 61, 377-392,
5. Isaac, O. O., Chinwe, J. A.. "The phytochemical analysis and antibacterial screening of extract of tetrecarpidum conophorum" 26(1): 53-55,
6. Khodursky, A.B. , Cozzarelli, N. R.. "The mechanism of inhibition of topoisomerase IV by quinolone antibacterials" 273, 27668-2767,
7. Maridass, M. . "Essential oils of ethnomedicine of diospyros malabarica fruits" ,
8. Maridass, M. . "Phytochemicals from genus diospyros (L.) and their biological activities" 12, 231-244,
9. Nonaka, G. , Nishioka, M. , Nishizawa, T. . "Anti-AIDS agents" 53, 587-590,
10. Obritsch, M. D., Fish, D.N. , MacLaren, R. , Jung, R. . "Nocosomial infections due to multidrug-resistant pseudomonas aeruginosa: epidemiology infection and treatment options" ,
11. Odebiyi, A. , Sofowora, A. E.. "Phytochemical screening of nigerian medicinal plants" 41, 243-246,
12. Phillipson, J. D., O, M. J.. "Medicinal plants against protozoal diseases" 85, 18-21,
13. Ragasa, C. Y.. "Triterpenes from diospyros blancoi" ,
14. Suresh, B. , Sriram, S. , Dhanaraj, S. , Elango, K. , Chinnaswamy, K. . "Anticandidal activity of santolina chamaecyparissus volatile oil" 55, 151-159,
15. Talaro, K. P., Talaro, A. . "Foundations in microbiology basic principles." , 97-98,
16. Watling, S. M., Dasta, J. F.. "Aminoglycoside dosing considerations in intensive care unit patients" ,
17. Arteca, R. . Plant growth subtances principles and applications. 29-31.
18. Hogg, S. . Essential microbiology. 353-372.
19. Kaufman, P. , Leland, C. , Warber, S. , Duke, J. , Brielmann, H. . Natural products from plants. 124-128.
20. Madigan, M. , Martinko, J. . Brock biology of microorganisms. 685.
21. Nester, E. , Anderson, D. , Roberts, E. , Persall, N. . Microbiology a human perspective. 518-519.
22. Poster, G. , Twell, D. . Plant gene isolation principles and practice. 46.
23. Prescott, L. , Harley, P. , Klein, D. . "Microbiology" , 783,
24. Quisumbing, E. . Medical plants of the Philippines. 936-937.
26. (). . Retrieved from: http://www.sitemaker.edu/mc9/gentamicin
31. (). . Retrieved from: http://www.stuartxchange.org/MAbolo.html
 

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