In vitro cytotoxicity and binding studies of anti-TAG72 immunoliposomes loaded with Doxorubicin

PHRR120920-000005

In vitro cytotoxicity and binding studies of anti-TAG72 immunoliposomes loaded with Doxorubicin

The project aims to show that anti-TAG72 immunoliposomes specifically bind then kill TAG72-expressing cells. The immunoliposomes are synthesized from commercial reagents (Dr. Sabido, Project 2). They are then loaded with Doxorubicin. A humanized anti-TAG72 antibody, hCC49 (Dr. Bascos, Project 1), is then attached to the liposome. The amount of radiation, Doxorubicin, and antibody per liposome capsule are consistent and known. Controls include naive liposomes. Specific binding is shown through competition binding in the presence of hCC49. Specific binding can also be shown and quantified by flow cytometry. Extent of cytotoxicity should correlate witth the amount of binding. Binding is followed through scintillation counting, incorporation by flow cytometry, and cytotoxicity by MTT metabolism. Autoradiography can also be done to show that the Tritium-labeled liposomes had entered the tumor cells.

Regime Classification Priority
2010 - 2016 Health Technology Development Drug Discovery and Development
Start Date Duration in Months Target Completion Date Actual Completion Date
2012-06-01 11 2013-05-01 0000-00-00

Ongoing

Institution Classification Region LTO #
University of the Philippines - Diliman, Institute of Biology Public Higher Education Institution - State Universities and Colleges NCR
Institution Region
Philippine Council for Health Research and Development, Department of Science and Technology NCR
Name E-Mail Institution and Institution Address
Dr. Sonia D. Jacinto soniajac2008@gmail.com Institute of Biology, University of the Philippines, Diliman, Quezon City
Name E-Mail Institution and Institution Address
Dr. Sonia D. Jacinto soniajac2008@gmail.com Institute of Biology, University of the Philippines, Diliman, Quezon City
Name Expertise Affiliation
Sonia D. Jacinto, PhD Development of transformed tissues, cell and enzyme-based assays to establish cancer chemotherapeutic and/or chemopreventive properties of plant and animal extracts or compounds University of the Philippines - Diliman, Institute of Biology
Name of Trial Site Address Principal Investigator Research Ethics Committee
NAME EXPERTISE REC Name Status of Approval
University of the Philippines - Diliman, Institute of Biology Institute of Biology, University of the Philippines - Diliman, Quezon City Sonia D. Jacinto, PhD Development of transformed tissues, cell and enzyme-based assays to establish cancer chemotherapeutic and/or chemopreventive properties of plant and animal extracts or compounds

Non-clinical Studies

Breast cancer

Unspecified

Unspecified

Unspecified

  • Philippines

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