Unspecified
In vitro cytotoxicity and binding studies of anti-TAG72 immunoliposomes loaded with Doxorubicin
PHRR120920-000005
In vitro cytotoxicity and binding studies of anti-TAG72 immunoliposomes loaded with Doxorubicin
The project aims to show that anti-TAG72 immunoliposomes specifically bind then kill TAG72-expressing cells. The immunoliposomes are synthesized from commercial reagents (Dr. Sabido, Project 2). They are then loaded with Doxorubicin. A humanized anti-TAG72 antibody, hCC49 (Dr. Bascos, Project 1), is then attached to the liposome. The amount of radiation, Doxorubicin, and antibody per liposome capsule are consistent and known. Controls include naive liposomes. Specific binding is shown through competition binding in the presence of hCC49. Specific binding can also be shown and quantified by flow cytometry. Extent of cytotoxicity should correlate witth the amount of binding. Binding is followed through scintillation counting, incorporation by flow cytometry, and cytotoxicity by MTT metabolism. Autoradiography can also be done to show that the Tritium-labeled liposomes had entered the tumor cells.
| Regime | Classification | Priority |
|---|---|---|
| 2010 - 2016 | Health Technology Development | Drug Discovery and Development |
| Start Date | Duration in Months | Target Completion Date | Actual Completion Date |
|---|---|---|---|
| 2012-06-01 | 11 | 2013-05-01 | 0000-00-00 |
Ongoing
| Institution | Classification | Region | LTO # |
|---|---|---|---|
| University of the Philippines - Diliman, Institute of Biology | Public Higher Education Institution - State Universities and Colleges | NCR |
| Institution | Region |
|---|---|
| Philippine Council for Health Research and Development, Department of Science and Technology | NCR |
| Name | Institution and Institution Address | |
|---|---|---|
| Dr. Sonia D. Jacinto | soniajac2008@gmail.com | Institute of Biology, University of the Philippines, Diliman, Quezon City |
| Name | Institution and Institution Address | |
|---|---|---|
| Dr. Sonia D. Jacinto | soniajac2008@gmail.com | Institute of Biology, University of the Philippines, Diliman, Quezon City |
| Name | Expertise | Affiliation |
|---|---|---|
| Sonia D. Jacinto, PhD | Development of transformed tissues, cell and enzyme-based assays to establish cancer chemotherapeutic and/or chemopreventive properties of plant and animal extracts or compounds | University of the Philippines - Diliman, Institute of Biology |
Non-clinical Studies
Breast cancer
Unspecified
Unspecified
- Philippines