The research was conducted to analyze the effect of saba as filtering agent against contaminated water with Escherichia coli. In particular, the study (1) described the use of water in our daily lives and saba as well, (2) discussed the level of awareness regarding contaminated water most especially by the bacteria E. coli, (3) analyzed which part of saba and what content of saba peels helped us to filter the bacteria, (4) identified the problems encountered in doing the whole experiment, (5) analyzed which concentrations (250g, 5005, 750g) is most effective to filter the contaminated water, (6) determined the financial viability of this study.
Physical and Phytochemical test were done after cutting, drying and pulverizing the peels of saba to identify which part of the saba and what is the content that can filter the bacteria. Escherichia coli was bought and tested at the National Institute of Molecular Biology and Biotechnology (BIOTECH) at the University of the Philippines Los Banos, Laguna. Also Mcfarland standard was used for the confirmatory of E. coli. Different concentration of 250, 500 and 750 grams were also done. Mcfarland standard was used as the positive control and distilled water as the negative control, each concentrations were mixed to 900 ml of water with 100 ml of E. coli. and let them stand for at least 20 minutes before filtering. Two different test were done first is the heterotrophic plate count and second is the multiple fermentation tube, we did three trials for this study to double and triple check for the results.
Based on the results of the study, the researcher also uses negative and positive controls to ensure the accuracy of the test results. Using E. coli Pour plate and the 9221-E multiple tube fermentation methods the count of organism in the water samples significantly lessen in each tower compare to the positive control. The banana peels may not completely filtered the E. coli organism nonetheless it helps to reduce the count of the organism.