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Submitted: 07 December 2015 Modified: 20 September 2017
HERDIN Record #: PCHRD15120704514674

Comparison of the in-vitro effects of bevacizumab, mitomycin-C, 5-fluorouracil,and triamcinolone acetonide on the viability of cultured human tenon's fibroblasts.

1Pius Jonas Ocampo,
2Ma. Margarita Lat-Luna,
3Joseph Anthony Tumbucon,
4Polly Chao-Po,
5Andrei Martin
International Eye Institute - St. Luke's Medical Center,
Department of Ophthalmology and Visual Sciences - Department of Obstetrics and Gynecology, Univeristy of the Philippines-Philippine General Hospital, UP-PGH,
Department of Ophthalmology - Cardinal Santos Medical Center

Abstract

Objective: To evaluate and compare the effects of bevacizumab, mitoinycin-C (MMC), 5-fluorouracil (5-FU), and triamcinolone acetonide (TA) on the viability of cultured human Tenon's capsule fibroblasts (cHTF) in vitro.


Methods: Human Tenon's fibroblasts (HTF) were harvested and cultured in a Roswell-Park-Memorial 1-Institute (RPMI) media. MMC, 5-FU, bevaciz. umab, and TA were administered to the cHTF at 3-fold decreasing concentrations starting from 20 ug, 5 mg, 25 mg, and 4 mg respectively. A negative control/untreated group containing RPMI media only was included in the study. Fibroblast cell viability was assessed using resazurin fluorim etric assay. Half¬maximal inhibitory concentration (IC50) was computed for agents which showed significant decrease in cHTF viability compared to the untreated group.


Results: There was no significant difference in cH IF viability between the untreated control group compared to 5-FU (p=0.97), bevacizumab (p=0.10), and TA (p=0.06) groups. Mitomycin-C showed a significant decrease in cHTF viability (p<0.001) which was dose dependent. The IC50 of MMC was computed at 12.16 ug using the prism software.


Conclusion: Mitomycin-C demonstrated dose-dependent decrease in viability of cultured human Tenon's fibroblasts. 5-FU, bevacizumab, and triamcinolone did not show this effect.


Key Words: Mitomycin-C, 5-fluorouracil, Bevaciz. umab, Tria. mcinolone acetonide, Fibroblast, Trabeculectomy

1.
Publication Type:
Journal
Publication Sub Type:
Journal Article, Original
Title:
Philippine Journal of Ophthalmology
Frequency:
Semi-Annual
Publication Date:
July-December 2014
Volume:
39
Issue:
2
Page(s):
84-89
Publisher:
Philippine Academy of Ophthalmology

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