Report an error   


Submitted: 18 September 2013 Modified: 17 May 2017
HERDIN Record #: PCHRD13091810515842

Prevalence of dengue infection and accuracy of the rapid dengue-NS1 antigen strip test as a screening tool for dengue infection among patients presenting with fever at the emergency room of St. Luke's Medical Center (A prospective study).

AnnaKristine A. Ramos,
Faith Joan C. Mesa-Gaerlan,
Peter F. Quilala,
Cynthia A. Mapua,
Lady-Anne C. Suarez,
Hannah Karen M. Labayo,
Maria Luisa G. Daroy,
Ronald R. Matias,
Filipinas F. Natividad

Dengue infection, an arthropod-borne disease spread by the Aedes aegypti mosquito, is a major cause of morbidity and mortality in the Philippines, where epidemics occur every 3 to 4 years and cases increase to as high as 88% every year. This study has determined the accuracy of the dengue-NS1 test as a screening tool for dengue infection among patients with fever for 1 to 3 days presenting at the emergency room of the St. Luke's Medical Center. After enrolling 147 participants, the study found out that the dengue-NS1 strip test had a sensitivity of only 20% when compared to IgM-capture ELISA and 23.4% when compared to PCR/ viral culture. Specificity, however, was high at 80% when compared to IgM-capture ELISA and 86.7% when compared to PCR/ viral culture. The dengue-NS1 strip test excludes the diagnosis of dengue infection 65 to 68% of the time and confirms it in only 43%.

Publication Type
Journal
Publication Sub Type
Journal Article, Original
Title
St. Luke's Journal of Medicine
Frequency
Semi-Annual
Publication Date
January-June 2012
Volume
8
Issue
1
Page(s)
31-38
LocationLocation CodeAvailable FormatAvailability
Philippine Council for Health Research and Development Library Fulltext pdf (Request Document)
1. Pei- Yun S, , Jyh-Hsiung H, . "Current Advances in Dengue Diagnosis" Clinical and Diagnostic Lahoratory Immunology , 642-650, 2004
2. Dengue Diagnostics: Proceedings of an International Workshop, 4.6 October 2004. Dengue Diagnostics and Dengue Classification! Case Management WHO/TDR Geneva, Switzerland
3. Daniel H, , Libraty, , et al., . "High Circulating Levels of the Dengue Virus Nonstructural Protein NS1 Early in Dengue Illness Correlate with the Development of Dengue Hemorrhagic Fever" The Journal ofInfectious Diseases , 1861165-8, 2002
4. Kalayanarooj S, . "Early Clinical and Laboratory Indicators of Acute Dengue Illness" The Journal of Infectious Diseases 176, 313-21, 1997
5. The Malaria, Other Vectorborne and Parasitic Diseases Unit of the Western Pacific Regional Office of the World Health Organization (WHO). Update of the Principles and Use of Rapid Tests in Dengue April 2009.
6. WHO: Impact of Dengue. 2009.
7. Sekaran SD, , Ew CL, , Subramaniam G, , Kanthesh BM, . "Sensitivity of dengue virus NS-1 detection in primary and secondary infections" African Journal of Microbiology Research 3(3): 105-110, March 2009
8. Gubler, D J. . "Dengue and Dengue Hemorrhagic Fever Clinical and Microbiological Reviews" , 480-496, July 1998
9. Vaughn, David , et al., . "Evaluation of a Rapid Immmunochromatographic Test for Diagnosis of Dengue Virus Infection" Journal of Clinical Microbiology , 234-238, January 1998
10. World Health Organization. Dengue Hemorrhagic Fever: diagnosis, treatment, prevention, and control 1997.
11. Gibbons, , et al., . "Dengue as an escalating problem. " British Medical Journal 324, 1563-1566, 2002
12. Morita, , et al., . "Rapid Identification of Dengue Virus Serotypes by Using Polymerase Chain Reaction" Journal of Clinical Microbiology , 2107-2110, October 1991
13. Dussart, , et al., . "Evaluation of an Enzyme Immunoassay for Detecction of Dengue Virus NS1 Antigen in Human Serum" Clinical and Vaccine Immunology 13(11): 1185-1189, November 2006
14. Ludert et al., . "Use of a commercial enzyme immunoassay to monitor dengue virus replication in cultured cells" J Virology Journal , April 2008
15. Hang VT, , Nguyen D, , et al., . Diagnostic Accuracy of NS1 ELISA and Lateral Flow Rapid Tests for Dengue Sensitivity, Specificity and Relationship to Viraemia and Antibody Responses. Available at: www.plosntds.org. Jan 2009.
16. Kumarasamy V, , Wahab AH, , Chua SK, , et al., . "Evaluation of a commmercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection" JVirolMethods 140(1-2): 75-9, March 2007
17. Zainah S, , Wahab AH, , et al., . "Performance of a commercial rapid dengue NS1 antigen immunochromatography test with reference to dengue NS1 antigen-capture ELISA" JVirolMethods 155(2): 157-60, February 2009
18. Chaiyaratana W, , et al., . "Evaluation on nonstructural protein 1 antiigen strip for the rapid diagnosis of patients with dengue infection" Diagnostic Microbiology and Infectious Disease , 83-84, May 2009
19. Ramirez A, , et al., . "Evaluation of dengue NS1 antigen detection tests with acute sera from patients infected with dengue virus in Venezuela" Diagnostic Microbiology and Infectious Disease , 247-253, November 2009
20. Datta S, , Wattal C, . "Dengue NS1 antigen detection: A useful tool in early diagnosis of dengue virus infection" Indian J Med Microbiol 28, 107-10, 2010
21. Libraty DH, , Young PR, , Pickering D, , Endy TP, , Kalayanarooj S, , Green S, , et al., . "High circulating levels ofthe dengue virus nonstrucctural protein NS1 early in dengue illness correlate with development of dengue haemorrhagic fever" J Infect Dis 186, 1165-8, 2002

Copyright © One Window Project 2018. All rights reserved.