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Submitted: 22 August 2012 Modified: 04 May 2017
HERDIN Record #: NCR-RITM-12082212491833

Molecular detection and serotyping of dengue virus in Metro Manila, Philippines.

1Ronald R. Matias,
2Ma. Giselle V. Espiritu,
3Corazon C. Buerano,
4Filipinas F. Natividad,
5Akira Igarashi,
Institute of Biology/College of Science - University of the Philippines,
Research and Biotechnology Division - St. Luke's Medical Center,
Department of Molecular Epidemiology - Institute of tropical Medicine Nagasaki University,
Department of Virology - Institute of tropical Medicine Nagasaki University

Abstract

Dengue virus infection is a persistent health problem in the Philippines. Surveillance and diagnosis have been limited to recognizing the clinical symptoms. In this study, a reverse transcription-polymerase chain reaction (RT-PCR) assay complemented with virus isolation was used to detect and isolate dengue virus from sera of patients admitted to different medical institutions during the period 1995-1998. RT-PCR was done directly on serum samples. Additionally, serum samples were inoculated on Aedes albopictus cells and RT-PCR was done on infected culture fluid or on RNA extracted from infected cells. Dengue virus was detected and isolated in 95 out of 738 serum samples. Serotyping of the virus indicated the prevalence of Den 2 and Den 3.

1.
Publication Type:
Journal
Publication Sub Type:
Journal Article, Original
Title:
National Academy of Science and Technology Philippines.
Frequency:
Monthly
Publication Date:
Page(s):
0
Publisher:
National Academy of Science and Technology Philippines.

2.
Publication Type:
Journal
Publication Sub Type:
Journal Article, Original
Title:
St. Luke's Journal of Medicine
Frequency:
Bimonthly
Publication Date:
January-June 2010
Volume:
6
Issue:
1
Page(s):
83-85
Publisher:
St. Luke's Medical Center

LocationCall NumberAvailable FormatAvailability
RITM Fulltext Print Format
Philippine Council for Health Research and Development Library Box No. 35 Fulltext pdf (Request Document)
1. Deubel, V , Kinney, R. M., Trent, D. W.. "Nucleotide sequence and deduced amino acid sequence of the structural protenins of dengue type 2 virus, Jamaica genotype." Virology 155, 365, 1986
2. Hahn, Y. S., Galler, R. , Hunkapiller, T. , Dalrymple, J. M., Strauss, J. M., Strauss, E. G.. "Nucleotide sequence of dengue 2 RNA and comparison ofthe encoded proteins with those of Flaviviruses." Virology 162, 167-180, 1988
3. Henchal, E. , Putnak, J. R.. "The dengue viruses" Clinical Microbiology Reviews 3, 376-396, 1990
4. Igarashi, Akira . "Isolation of a Singh's Aedes albopicuts cell clone sensitive to dengue and chikungunya viruses." Journal of Virology 46, 534-544, 1978
5. Lanciotti, R. S., Calisher, C. H., Gubler, D. J., Chang, G. J., Vorndam, A. V.. "Rapid detection and typing of dengue viruses form clinical sample using reverse transcriptase-polymerase chain reaction." Clinical Microbiology 30, 545-551, 1992
6. Mackow, E. Y., Makono, B. , Zhao, Y. M., Zhang, L. , Markoff, A. , Buckler-White, M. , Guiler, R. , Chanock, , Lai, C. . The nucleotide sequence ofdengue type 4 virus: analysis ofgenes coding for nonstructural protein.. Virology: 159, 217-228
7. Mason, P. W., McAda, P. C., Mason, T. L., Fournier, M. J.. "Sequence ofthe dengue-l virus genome in the region encoding the three structural proteins and the major nonstructural protein NSI" Virology 161, 262-267, 1987
8. Osatomi, K. , Sumiyoshi, H. . "Complete nucleotide sequence type 3 virus genome RNA." Virology 176, 643-647, 1990
9. Rice, C. M., Lenches, E. M., Eddy, S. E., Shin, S. J., Sheets, R. L., Strauss, J. H.. Molecular cloning of flavivirus genomes for comparative analysis and expression. In: H. Bauer, H. D. Klenk and C.. Scholtissek (Eds) Trends in Virology Berlin: Springer-Verlag, 1988. 83-98.
10. Rolfs, A. , Scheller, I. , Finckh, U. , Weber-Rolfs, I. . PCR Clinical Diagnostics and Research.. Berlin: Springer-Verlag, 1992. 271.
11. Seah, C.L. K., Chow, V.T. K., Chan, Y. C.. "Semi-nested PCR using NS# primers for the detection and typing of dengue viruses in clinical serum specimens." Clinical and Diagnostic Virology 4, 113-120, 1995
12. Zhao, B. E., Buckler-White, A. , Mackow, E. , Markoff, L. , Chanock, R. M., Lai, C. J., Makino, Y. . "Cloning full-length dengue type 4 viral DNA sequences: analysis ofgenes coding for structural proteins." Virology 155, 77-88, 1986