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Submitted: 05 July 2018 Modified: 25 April 2019
HERDIN Record #: R07-CIM-18070517594712

The anti-inflammatory effect of Averrhoa bilimbi leaf extract on croton-oil induced inflammation of ICR mice ears.

Streffi Jasmyn Belderol,
Patrick Louis Cabaaero,
Nestor Camarinta,
Sim Vincent Fernandez,
Norman Formilleza Jr.,
Lou Angelie Gallares,
Dayanara Mae Garcia,
Charize Marian Gulfan,
Clarenz Marie Jaranilla,
Niña Rica Lim,
Rebecca Maguad,
Jaya Mishra,
Hamsar Pagayao,
Bianca Patangan,
Kimbely Nicole Yu

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Rationale: Averrhoa bilimbi is a plant common in Asia, which has been historically used as treatment for inflammation and numerous reports have claimed it has anti-inflammatory properties. Furthermore, it has phytochemicals such as flavonoids, alkanoids and phenols, which have been reported to have anti-inflammatory properties. Studying its anti-inflammatory effects may pave the way to the development of alternative treatment for inflammation.Objective: To determine the potential topical anti-inflammatory effect of Averrhoa bilimbi leaf extract on croton oil induced inflammation in ICR Mice ears. Methodology: Crude extract from A. bilimbi leaves was extracted via ethanol extraction. Male ICR mice were randomly sorted into seven groups. Ear thickness of mice was measured using a vernier caliper. Ear edema was induced by topical application of croton oil. Treatment was applied an hour later, according to grouping. Test groups were treated with different doses of the crude extract dissolved in petroleum jelly (wt/wt%), which was 30%, 60%, and 90% respectively. Ear thickness was measured every hour for 6 hours after induction of inflammation. Pain score and erythema were also measured. Positive and negative control groups were treated with Ketoprofen and Petroleum jelly respectively. Ear thickness and erythema will be evaluated using grading scales for descriptive purposes. Results: After 6 hours, the mean ear thickness of the positive control (0.16um), 90% group (0.16um) were significantly lower than the 30% group  (0.22um) and the negative control (0.23um). IC50 is -73.4%. Pain score decreased in the positive control (-1.62), 90% group (-1.38), 60% group (-0.64) and negative control (-0.64) while it increases for the 30% group (0.74). Only negative control had a  significantly decreased erythema score from 2.1 to 0.3. Conclusion: A. bilimbi leaf extract has a topical-inflammatory effect against croton oil induced inflammation in ICR mice ears. It is capable of decreasing ear swelling and effectiveness is increased with dosage. However, it does not decrease erythema and effect on pain score is still unclear. Recommendations: Further studies need to be done with better assessment tools and with other anti-inflammatory models. Phytochemical screening should be done to determine the phytochemical responsible for its anti-inflammatory effect.

Publication Type
Thesis/Dissertations
Thesis Degree
MD
Specialization
Medicine
Publication Date
2016
LocationLocation CodeAvailable FormatAvailability
Cebu Institute of Medicine Abstract Print Format
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