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Submitted: 09 December 2015 Modified: 09 December 2015
HERDIN Record #: CAR-UCBCF-15120901204314

Extended disinfection time reduces bacteriostatic efficacy of contact lens cleansing solutions against bacterial contaminants.

Edzel Chelsea Estrella,
Madison Munar,
Jaymee Oclarit

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Bacterial keratitis is a common eye infection case of contact lens
users worldwide. This eye infection is caused by exposure to bacterial
contaminants that grow due to unhygienic practices and irregular
disinfection of the contact lens and its cases. This study has shown that
Staphylococcus aureus and Staphylococcus epidermis were among the
common bacterial contaminants on contact lens cases. 16S rRNA
sequencing of unknown bacterial isolates revealed the presence of other
bacterial species such as Escherichia vulneris and Bacillus subtilis, a gram
negative and a gram positive bacterium, respectively. The bacteriostatic
efficacy of two commonly used multipurpose disinfecting solutions
was investigated in this study. It was observed that bacteriostatic
efficacy of the two disinfecting solutions was reduced as observed in the
increased colony forming units on 24 hours and 48 hours extended
disinfection time. Moreover, disinfection of contact lens cases without
hand washing significantly increased the number of colony forming

units as revealed by Paired T-test at 5% level of significance.


Publication Type
Others
Place of Publication
Research Journal of Pharmaceutical, Biological and Chemical Services
Publication Date
2015

Objectives













This study has shown that
Staphylococcus aureus and Staphylococcus epidermis were among the
common bacterial contaminants on contact lens cases.