The researchers decided to find an alternative stain which is less expensive and less hazardous to the health. The study utilized a randomized posttest only experimental control groups to determine the potential of Alugbati (Basella alba) fruit and Mangosteen (Garcinia mangostana) pericarps extractives as substitute for Crystal Violet and Safranin in Gram staining technique. Ninety-five percent (95%) ethanol was used to extract juice from 200 grams of Mangosteen pericarps and 150 grams of Alugbati fruit. Eight slides were smeared with Staphylococcus aureus and eight slides were smeared with Escherichia coli. When using the Gram staining procedure, none of the slides were stained with Alugbati (Basella alba) as an alternative for Crystal Violet and Mangosteen pericarps (Garcinia mangostana) as alternative for Safranin. However, when extracts of Alugbati fruit and Mangosteen pericarps were used solely, it faintly stained both Staphylococcus aureus and Escherichia coli.
The findings of the study were presented, analyzed and interpreted in this chapter in four (4) sections; the number of slides of Staphylococcus aureus stained with Alugbati fruit extractive as compared to Crystal Violet, the number of slides of Escherichia coli stained with Mangosteen pericarps extractive as compared to Safranin, a significant difference between the proportions of slides positive for staining of Staphylococcus aureus using Alugbati fruit extractive and Crystal Violet, a significant difference between the proportions of slides positive for staining of Escherichia coli using Mangosteen pericarps extractive and Safranin. No slides were stained with Alugbati fruit extractive and Mangosteen Pericarps extractive.
Based on the findings, Alugbati fruit extractive and Mangosteen pericarps did not have the potential as staining agents on bacteria. The researchers also recommend that modifications to the Gram staining technique should be considered when using alternative stains.