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Submitted: 13 January 2015 Modified: 22 August 2019
HERDIN Record #: NCR-UERMMMC-1501130433512

Laboratory observations on the use of Diplonychus rusticus as a potential biological control agent for Japanese encephalitis vector.

Nelia P. Salazar

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Introduction This study explored the practical use of the local aquatic bug Diplonychus rusticus in the laboratory as a potential biological control agent for the larvae of the vectors of Japanese encephalitis, Culex tritaeniorhynchus and Culex vishnui, which are commonly found in rice fields.


Methods Diplonychus rusticus was reared in the laboratory and its reproductive behaviour and longevity were observed. Single predators consisting of III instar and adult female, respectively, were each placed in two containers with 100 Culex vishnui instar each as prey. The water bugs' feeding pattern were observed for 24 hours.


Results Diplonychus rusticus underwent five nymphal instar stages with an average developmental period of 50 days from egg to adult. The adult female laid six egg batches with 30 to 65 eggs per batch. The adult female lived up to 80 days while the adult male lasted 103 days. With 100 III instar and IV instar larvae of Culex vishnui given as prey, the III instar nymph of Diplonychus rusticus consumed 98% of the prey in 24 hours while the adult female consumed 96% of the prey in 12 hours and 100% at the end of 24 hours.


Conclusion Diplonychus rusticus is a potential biological control agent for the mosquito vector of Japanese encephalitis Culex vishnui.

Publication Type
Journal
Publication Sub Type
Journal Article, Original
Title
The Health Sciences Journal
Frequency
Others
Publication Date
January-June 2014
Volume
3
Issue
1
Page(s)
19-24
LocationLocation CodeAvailable FormatAvailability
UERMMMC Fulltext Print Format
1. World Health Organiz, . Handbook for integrated vector management Geneva: World Health Organization , 2012.
2. Lacey, L A, Orr, B K. "The role of biological control of mosquitoes in integrated vector control" Am J Trop Med Hygiene 50(6 Suppl): 97-115, 1994
3. World Health Organiz, . Manual on environmental management for mosquito control Geneva: WHO Offset Publication 66, 1982.
4. Kumar, R , Hwang, JS . "Larvicidal efficiency of aquatic predators: a perspective for mosquito biocontrol" Zoological Studies 45(4): 447-66, 2005
5. Shalaan, EA-S , Canyon, D V. "Aquatic insect predators and mosquito control" Tropical Biomedicine 26(3): 223-61, 2009
6. Sivagnaname, N . "A novel method of controlling a dengue mosquito vector Aedes aegypti (Diptera: Culicidae) using an aquatic mosquito predator, Diplonychus indicus (Hemiptera: Belostomatidae) in tyres" Dengue Bulletin , 148-60, 2009
7. Javier, P A, Albaytar, A B, Javier, P A, Albaytar, A B. Laboratory evaluation of water bug Diplonychus rusticus (F) (Hemiptera: Belotomatidae) for the control of dengue mosquito, Aedes aegypti Linnaeus (Diptera: Culcidiae). Los Banos: UPLB, 2012.
8. Javier, P A, Albaytar, A B, Javier, P A, Albaytar, A B. Laboratory evaluation of water bug Diplonychus rusticus (F) (Hemiptera: Belotomatidae) for the control of dengue mosquito, Aedes aegypti Linnaeus (Diptera: Culcidiae). Los Banos: UPLB, 2012.
9. Varela, R P, Gapud, V P, Degamo, J R, Varela, R P, Gapud, V P, Degamo, J R. Aquatic insect diversity in the lentic region of Agusa Marsh.
10. Varela, R P, Gapud, V P, Degamo, J R, Varela, R P, Gapud, V P, Degamo, J R. Aquatic insect diversity in the lentic region of Agusa Marsh.
11. Sivagnaname, N , Sivagnaname, N . Biocontrol potential of Diplonychus indicus Venk. 8 Rao (Hemiptera: Belostomatidae) against mosquito vectors with particular reference to the control of Aedes aegypti (Diptera: Culicidae). Pondicherry: Vector Control Research Center, 2000.
12. Sivagnaname, N , Sivagnaname, N . Biocontrol potential of Diplonychus indicus Venk. 8 Rao (Hemiptera: Belostomatidae) against mosquito vectors with particular reference to the control of Aedes aegypti (Diptera: Culicidae). Pondicherry: Vector Control Research Center, 2000.
13. Chandramohan, G , Arivoli, S , Venkatesan, P , Chandramohan, G , Arivoli, S , Venkatesan, P . "Effect of salinity on the predatory performance of Diplonychus rusticus (Fabricus)" Journal of Environmental Biology , 287-90, 2008
14. Chandramohan, G , Arivoli, S , Venkatesan, P , Chandramohan, G , Arivoli, S , Venkatesan, P . "Effect of salinity on the predatory performance of Diplonychus rusticus (Fabricus)" Journal of Environmental Biology , 287-90, 2008
15. Aditya, G , Bhattacharyya, S , Kundu, N , Kar, P K, Saha, G K, Aditya, G , Bhattacharyya, S , Kundu, N , Kar, P K, Saha, G K. "Predatory efficiency of the sewage drain inhabiting larvae of Toxorhynchites spendens Wiedemann of Culex quinquefasciatus Say and Armigeres sulbalbatus (Coquillett) larvae" Southeast Asian J Trop Med Public Health , 799-807, 2007
16. Aditya, G , Bhattacharyya, S , Kundu, N , Kar, P K, Saha, G K, Aditya, G , Bhattacharyya, S , Kundu, N , Kar, P K, Saha, G K. "Predatory efficiency of the sewage drain inhabiting larvae of Toxorhynchites spendens Wiedemann of Culex quinquefasciatus Say and Armigeres sulbalbatus (Coquillett) larvae" Southeast Asian J Trop Med Public Health , 799-807, 2007
17. Sivagnaname, N , Sivagnaname, N . "Selective and frequency dependent predation of aquatic mosquito predator Diplonychus indicus Venkatesan 8 Rao (Hemiptera: Belostomatidae) on immature stages of three mosquito species" Entomol Res , 356-63, 2009
18. Sivagnaname, N , Sivagnaname, N . "Selective and frequency dependent predation of aquatic mosquito predator Diplonychus indicus Venkatesan 8 Rao (Hemiptera: Belostomatidae) on immature stages of three mosquito species" Entomol Res , 356-63, 2009