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HERDIN Record #: NCR-UERMMMC-15010906185417 Submitted: 09 January 2015 Modified: 22 August 2019

Transovarial transmission of dengue virus in Aedes aegyti in Quezon City.

Nelia P. Salazar

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Introduction Due to the changing nature of dengue epidemiology and control, this study was conducted to determine and describe evidence of transovarial transmission of the dengue virus.

Methods This was a quantitative experimental study on the transovarial transmission of the dengue virus from field-collected Aedes aegypti in an animal model. Viremia was detected by reverse transcriptase-polymerase chain reaction. Mosquito homogenate was used for intracranial inoculation of the virus into suckling mice. The brains of the suckling mice were extracted and inoculated intraperitoneally into 3 to 4 week old mice for recording of disease manifestation. 

Results The mice infected intraperitoneally with dengue virus from field Aedes mosquitoes showed evidence of dengue disease manifested through physical signs, thrombocytopenia and histopathologic changes in affected organs.

Conclusion These observations indicate that transovarial transmission of dengue virus can occur in a highly urbanized locale like Quezon City where dengue cases are high, and virulence may translate into dengue disease when inoculated in an animal model.

Publication Type
Publication Sub Type
Journal Article, Original
The Health Sciences Journal
Publication Date
January-June 2014
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