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Submitted: 11 July 2013 Modified: 09 March 2019
HERDIN Record #: R07-CDU-13071116430323

The antimicrobial effect of 60% psidium guajava (Guava) leaf juice and 60% imperata cylindrica (Cogon) leaf juice against escherichia coli in vitro.

Angelito Prieto,
Katrina R. Cebrian,
Cesar George C. Cumba,
Therese Frances C. Ferandos,
Katherine M. Liao,
Nestor T. Nipangue Jr.,
Nausica A. Pepito,
Ellie O. Sia,
Mahalia M. Vidal

Objective: To compare the animicrobial effect of 60% Psidium guajava leaf juice and 60% Imperata cylindrica leaf juice against Escherichia coli in vitro.


Design: Experimental design.


Setting: The Cebu Doctors' University Hospital Out-patient Microbiology laboratory located in the ground floor of the hospital building.


Study Subjects: Escherichia coli culture from CDUH Out-patient Microbiology laboratory with stock number CDUH-06011


Procedures or Interventions: The Kirby-Bauer Disk Diffusion method was used in the study. Seven Mueller Hinton Infusion Agar plates were inoculated with Escherichia coli. A disk impregnated with 60% Psidium guajava leaf juice, another with 60% Imperata cylindrica leaf juice and a Piperacillin-Tazobactam antibiotic disk where placed in each of the 7 plates. The inoculated plates where incubated in the incubator at 37 degree celcius for 16-18 hours. The zones of inhibition made by the disks were the measured using a vernier caliper. The mean zone of inhibition with its corresponding standard deviation was computed Analysis of Variance and Duncans' Multiple-Range Test was utilized to determine if a significant difference exists in the zones of inhibition among the samples.


Research Instrument: A vernier caliper was used to measure the zone of inhibition produced by the different disks impregnated with 60% Psidium guajava leaf juice, 60% Imperata cylindrica leaf juice and Piperacillin-Tazobactam against Escherichia coli. A record sheet, devised by the researchers, was used to contain the following data: 1) Specimen number 2) Measurement of the zones of inhibition produced by the disks impregnated with 60% Psidium guajava leaf juice 3) Measurement of the zones of inhibition produced by the disks impregnated with 60% Imperata cylindrica leaf juice 4) Measurement of the zones of inhibition produced by the disks impregnated with Piperacillin-Tazobactam.


Results: Piperacillin-Tazobactam disk had a mean zone of inhibition of 21.71mm and a Standard Deviation of 0.88. The 60% Psidium guajava leaf juice disk had a mean zone of inhibition of 17.57mm and a Standard deviation of 1.05. The 60% Imperata cylindrica leaf juice disk had a mean zone of inhibition of 15.24mm and a Standard Deviation of 1.39. Comparison of the mean zone of inhibition among the three is significant difference in the mean zone of inhibition among the samples and between groups.


Conclusion: The 60% psidium guajava leaf juice and 60% Imperata cylindrica leaf juice both showed antimicrobial activity against Escherichia coli. The 60% psidium guajava leaf juice is more effective than 60% Imperata cylidrica leaf juice against Escherichia coli. piperacillin-Tazonbacta was the most effective among the three groups.

Publication Type
Thesis/Dissertations
Thesis Degree
MD
Specialization
Medicine
Publication Date
March 2006
LocationLocation CodeAvailable FormatAvailability
Mandaue City M An31 Abstract Print Format
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