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HERDIN Record #: R04A-DLSHSI-12100914521252 Submitted: 09 October 2012

Nine hour urine collection for protein quantification in hypertensive gravida and puerpera. A preliminary report.

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DESIGN This research is a prospective correlation analysis utilizing sequential, self-controls. The estimated 24-hour urine protein derived from proteinuria in a 9-hour urine collection (dependent variable) was correlated with the value obtained from a 24-hour urine collection (dependent variable).

PARTICIPANTS Twenty subjects were enrolled in the study, patients coming from both the De La Salle University Medical Center and Dr. Jose P. Rizal Hospital. Study period was from July 2000 up to September 2, 2000.

METHODS Indwelling foley catheterization was required of all patients enrolled. Time of collection was started at the time that initial urine was discarded. At the end of the 9th hour of collection, urine was sent to the laboratory for measurement of total volume and protein determination done from an aliquot. Urine was then refrigerated/ stored. The collection was continued until the 24th hour. Urine from the 9-hour collection was admixed to the urine collected during the consecutive 15 hours to obtain 24-hour urine volume. An aliquot was again obtained for protein determination. The extrapolated 24-hour urine protein excretion was derived from multiplying the factor 2.67 to the protein quantified in 9 hours. Correlation was determined using the Pearson's product-moment coefficient, Student's ? test and linear regression.

RESULTS The extrapolated nine hour urine collection had a significant positive correlation with the 24 hour urine collection (r = .9396, p <.000). Regression analysis revealed equivalent results (r=.93968, p <.000) and good model fit to the study data gathered.

CONCLUSIONS In this preliminary report, it has been shown that the nine-hour urine collection for quantitative protein determination is sufficient to predict the actual protein determination in 24 hours. However, mistaken bias in collection of study population resulted in ability of this study to evaluate better the extrapolated values and actual protein in diagnosed gestational and chronic hypertensives. The limited number of study enrollees may be deemed insufficient to draw stronger conclusions to support the merit of this research. It is proposed that any future investigation in the like of timed urine collection be done under a financial grant for budget, and conducted over a longer time period of data collection.


To assess whether a nine-hour collection is sufficient for quantitative analysis of protein excretion in the hypertensive pregnant woman and puerpera.