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HERDIN Record #: PCHRD090208020926 Submitted: 03 September 2008 Modified: 20 February 2019

Preliminary characterization of 3 pseudomonas bacteriophages from the new 6KZ-supergroup.

H W. Ackermann,
DMA dela Cruz,
S V. Krylov,
V N. Krylov

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Three bacteriophages active on Pseudomonas putida var. Manila and Pseudomonas fluorescens assigned to the 6KZ supergroup were isolated form soil and water samples from La Union province and Pasig River. Bacteriophages Lu11 and its smaller counterpart, Lu 11 .x belongs to the Family Podoviridae and infects P. fluorescens while phage P10 which infects P. putida var. Manila and belongs to the Family Myoviridae. Demonstrated here is the comparison of their resistance and sensitivity to different physical and chemical agents, plaque morphology, lysis of broth culture, graphical illustrations, similarity in morphotype and size of their particles based on SDS-PAGE electrophoresis. Using the Agar Layer Method, Lu11, having the biggest plaque size measured 3.0-3.5 mm, and P10 as the smallest, ranged 0.3-0.35 mm in diameter. Heat treatment showed all three phages inactivated at 60% while at different pH levels, ineffectivity of P1 o was stable at pH 4-10 and Lu11 phages only withstood pH 6-8. Phage P10 was stable when treated with 5,10 and 15% chloroform, Lu11 phages decreased their capacity to form plaques after 24h exposure to the agent. All three phages were tailed, belonging to the viral group having the most complex symmetry. Banding patterns of capsid proteins indicated Phage Lu11 closely related to other phages infecting P. aeruginosa while Phages P10 and Lu11.x with much fewer protein bands, are related to each other. Results based on this pioneering study open the doors to the classification and characterization of indigenous bacterial viruses in the Philippines. (Author)

Publication Type
Proceedings
Publication Sub Type
Symposium
Title
Philippine Network of Microbial Culture Collection
Date
Page(s)
13
LocationLocation CodeAvailable FormatAvailability
Philippine Council for Health Research and Development Library PR PNMCC Nov 18 2006 Abstract Print Format (Request Document)