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Submitted: 16 May 2007 Modified: 02 August 2012
HERDIN Record #: PCHRDHE890524

Cryptosporidial diarrhea: isolation and identification of causative organisms.

 Saniel MC,
 Gonzaga N,
 Laurente MC,
 Renejane MS,
 Geronimo JGI,
 Sabordo NT,
 Miranda MEG,
 Montalban CS

Cryptosporidium, a natural parasite of animals, was recognized as a cause of diarrheal disease in man in 1976. Recently, it has been reported to one of the organisms associated with opportunistic infections in acquired immune deficiency syndrome (AIDS). Cryptosporidial diarrhea is now a known human disease entirely throughout the world. It may be self-limiting or may be a part of a potentially fatal disease syndrome. Cryptosporidiosis in the Philippine children was first reported by Cross et al. in 1985. This paper reports the confirmation of cryptosporidiosis demonstrated on routine stool examination, through experimental infection in mice and by electrone microscopy. Following isolation and identification of oocystes from the stools of diarrheic patients by iodine and modified DMSO-carbol fuchsin staining methods, samples were concentrated by a sugar flotation technique and prepared for animal inoculation and electronmicrography. Experimental studies at the Research Institute for Tropical Medicine indicated: 1) the ability of human isolates to produce patent infection in BALB/c suckling and adult mice, 2) the ileum as the principal site of infection and, 3) presence of a definite organelle of attachment to the intestinal epithelial cell. Cryptosporidiosis of acute should be considered in the differential diagnosis of acute diarrhea in children and debilitated of immunocompromised adults. The etiology is confirmed by a 3-step procedure and by light microscopic examination

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