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Submitted: 12 January 2011 Modified: 05 April 2017

Herdin Record #: PCHRD01121101013764

Preliminary screening of cytotoxic activity of Raphanus sativus L. seeds and Schefflera odorata B. leaves and roots on different human-derived cancer cell lines.

Researchers

NameRole
1Josephine K. Sarau Author

Related Institutions

Institutions NameRole
University of Santo Tomas Authors Affiliation

Publication Information

1.
Publication Type:
Thesis/Dissertations
Thesis Degree:
MS
Specialization:
Pharmacy
Publication Date:
October 1997

Abstract

Fresh seeds of Raphanus sativus L. and air-dried leaves and roots of Schefflera odorata B. were extracted with 95% ethanol by percolation and fractionated with chloroform and water. Extracts after concentration were tested for its cytotoxicity activity on five cell lines, namely: A549 (adenocarcinoma of the lung), SL-6 (large cell of the lung), Calu-1 (squamous carcinoma of the lung), Hep-2 (epidermoid carcinoma of the larynx) and L929 (mouse fibroblast) used as negative control. Collaborative work on the lima-lima leaves and root extracts were done on other cell lines by Prof. Cordell of University of Illinois in Chicago. These cell lines were BCA-1 (breast cancer cells), LuC-1 (lung cancer cells), COL-1 (colon cancer cells), KB-3 (oral epidermoid carcinoma cells) and LN CaP, FGC, Crl (adenocarcinoma of the prostate). Taxol was used as the positive test control drug. After 3 days of exposure, cells were harvested, developed or assayed using MTT dye. From these data, inhibition concentration at 50% (IC50) values were derived for plant extracts which showed cell killing 50% or potential activity in each cell line. The results showed that Raphanus sativus L. alcoholic, chloroform and aqueous extracts were relatively non-cytotoxic to all cell lines tested at 1 to 200 ug/ml. The % cell survival ranges from 70 to 100% at 1 to 100 ug/ml drug concentration. Hence, it had a relatively high LD50 Schefflera odorata B. leaves and roots have IC5020 ug/ml for all cell lines tested with the extracts. At IC5020 ug/ml, the following relative sensitivity were established: alcoholic leaves extract to Calu-1 and A549, aqueous leaves extract to A549 at a relatively high dose, alcoholic roots extract to Calu-1, and chloroform roots extract to SL-6. Other extracts showed percentage cell survival 50% compared to other cell lines. IC50 of the cell lines tested by Prof. Cordell yield 20 ug/ml. Though, the extracts have exhibited relative sensitivity to some cell lines tested at a dose of 20 ug/ml it did not provide the level of cytotoxicity that is needed for a novel anti-tumor agents on the specific type of human tumor determined. The required dose or IC50 for a potential agent is set at 20 ug/ml as established by the American National Cancer Institute. However, it should be noted that this study refers to direct cell killing as the in vitro anti-tumor screening method and mechanism of action. Studies using other molecular mechanism of cytotoxic action and in vitro or in vivo screening procedure, are recommended. Further cytotoxicity tests are also recommended especially on other types of cell lines derived from other human cancer as sensitivity due to selectivity could be a factor.(Author)


Physical Location

LocationCall NumberAvailable FormatAvailability
Philippine Council for Health Research and Development Library RS164 Sa7 1997 Fulltext Print Format (Request Document)

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