Inflammation is a complex of cytologic and chemical reactions that occur in the affected blood vessels and adjacent tissues in response to injury or abnormal stimulation caused by physical, chemical, or a biologic agent. Several drugs were already formulated for their anti-inflammatory activity including guyabano which possesses different pharmacologic effects. They have been found to have antimicrobial, antiviral, anti-ulcerogenic, cytotoxic, mutagenic, antineoplastic, antioxidant, antihepatotoxic, antihypertensive, hypolidemic, antiplatelet, and anti-inflammatory action. The study tested the presence of flavonoids on the Guyabano Peel Extract which was responsible for the anti-inflammatory activity of the said extract. Furthermore, the study formulated an ointment and its anti-inflammatory activity was tested by Carrageenan-Induced Rat Paw Edema Test. A p-value of < 0.05 indicates that the difference wasnot significant whereas p-values at every interval greater than 0.05, indicates that the difference was not significant. Therefore, the anti-inflammatory activity of guyabano peel extract ointment was similar to the positive control, Indomethacin ointment. Sensitivity tests were also conducted to determine the safety of the formulated guyabano peel ointment when applied topically verifying that the formulated ointment is a non-irritant.