Makabuhay (Tinospora cordifolia) plant has been identified to be having antibacterial properties, giving rise to the formulated products such as ointments and soaps were which are now commercially available. This study aimed to determine the efficacy of Makabuhay plant extract which was formulated as a facial cleanser subjected to different concentrations of the plant extract (50%, 75%, and 100% concentrations). Its antibacterial property was tested against Staphylococcus aureus which is the causative agent of pimple and acne. The formulated Makabuhay facial cleanser was also compared to a commercially available facial cleanser (control). Makabuhay plant extract was extracted through percolation method. Assay plates were prepared for five trials and the antibacterial activity of Makabuhay plant extract was determined by Kirby-Bauer Test/ Disk-diffusion method. The test for the efficacy of the formulated facial cleanser against the control was determined by susceptibility testing using disk-diffusion method. A zone of inhibition (mm) was measured for every trial at different concentrations and was compared with the control facial cleanser. The formulated Makabuhay facial cleanser showed significant antibacterial activity against Staphylococcus aureus at 50% concentration, 75% concentration, and 100% concentration as the most effective. Notably, the control showed insignificant activity against Staphylococcus aureus. The experiment confirms the antibacterial property of Makabuhay (Tinospora cordifolia) plant extract as a formulated facial cleanser against Staphylococcus aureus at different concentrations.