Carrot (Daucuscarota) is known for its active constituent which is beta-carotene, but also has a wide diversity of being antioxidant, anti-cancer and even for cardiovascular diseases with respect to its nutritional content. It has also a powerful antiseptic agent for wounds. The researchers decided to explore the antibacterial activity of the fixed oil from carrot seeds. This study was conducted to determine the antibacterial activity of the formulated cream containing various concentrations (2%, 3%, 4% & 5%) of the fixed extracted from carrot seeds, employing Agar Cup Method. The results showed that the concentrations of formulated creams from Daucuscarota exerted antibacterial activity against Staphylococcus aureusas measured by the zone of growth of inhibition. Staphylococcus aureus was resistant to 2% to 3% concentrations as shown by its zone of growth of inhibition of 7.0000 and 12.6667 mm. Staphylococcus aureusshowed to be intermediate as shown by the zone of growth of inhibition of 16.3333 mm. The result of the study revealed that the 5% concentration of the formulated cream exerted the widest antibacterial activity as shown by the zone of growth of inhibition of 22.6667 mm and is comparable to the antibacterial activity of the positive control Mupirocin (Bactroban®) against Staphylococcus aureus. This underscores the need for further development of the study to perform safety test on the fixed oils, and conduct further studies regarding the potential antibacterial activity of the fixed oil from Daucuscarotato other gram-positive and gram-negative microorganisms and formulate the fixed oil in other dosage forms.