The study determined the wound healing effect of Jasminum sambac (Sampaguita) flower ethanolic extract among male Mus musculus (Albino mice). Experimental method was used in the study. The study was conducted in a a private residence located in Catarman, Lilo-an, Cebu. Ethanolic extract of J. sambac flowers was obtained. A wound was incised on each mouse of the control and experimental groups. The experimental group was treated topically with the J. sambac flower ethanolic extract, while the control group was treated with Normal Saline Solution (NSS). The wound size (in millimeters) of the study groups was determined by the use of a Vernier caliper and recorded daily for seven days. One-tailed t-test for independent samples was used to determiner the significant difference between the mean wound size of the experimental group and control group. The computer value of t at 95% level of confidence was -3.4797, which is less than the tabulated t value of -1.860. This suggests that there was a significant difference between the mean wound size of the said groups.