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Submitted: 27 March 2017 Modified: 27 March 2017
HERDIN Record #: R10-MSUIIT-17032710072975

GC-MS analysis and evaluation of in vitro antioxidant potential and total phenolics content of wild hops (Flemingia strobilifera.

Jhoan Rhea L. Pizon,
Olga  M.  Nuñeza,
Mylene M. Uy ,
W.T.P.S.K Senarath

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Wild hops (Flemingia strobilifera  Linn.)  is  a  shrub  belonging to Fabaceae  family.  The  leaves  of  F. strobilifera are commonly used by the Subanen, the indigenous group in Lapuyan, Zamboanga del Sur, Philippines to treat inflammation.  In  this  study,  the  hydromethanolic  (80%)  and  aqueous  leaf  extracts  of  F.  strobilifera  were evaluated for their antioxidant activity and total phenolics content. The active semi-volatile components of 80% methanol  leaf extract  were  analyzed  using  Gas Chromatography-Mass  Spectrometry  (GC-MS). DPPH  radical scavenging activity was used to determine the potential of this plant as anti-oxidant. Total phenolics content was determined using Folin-Ciocalteu reagent and calculated as gallic acid equivalence. GC-MS analysis revealed the presence of eight compounds. Both the 80% methanol and aqueous extracts of F. strobilifera showed significant scavenging  activity  with low  IC50  values  of 0.299  mg/mL  and <0.25  mg/mL,  respectively. There  was  positive correlation  between  the  scavenging  activity  percentage  and  the  total  phenolics  content  only  in  the  aqueous extract of F. strobilifera while 80% methanol extract showed negative correlation between inhibition percentage and total  phenolics  content which  can be  attributed to  the solvent  used and  method used in  quantification  of phenolics. Nevertheless, the results suggest that these leaf extracts are potent  source of antioxidant compounds and may serve as natural anti-inflammatory agents. 

Publication Type
Journal
Title
International Journal of Biosciences
Publication Date
February 2016
Volume
8
Issue
1
Page(s)
25-32
LocationLocation CodeAvailable FormatAvailability
Office of the Vice Chancellor for Research and Extension, Mindanao State University-Iligan Institute Technology Fulltext External Link (View)

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