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Submitted: 30 April 2014 Modified: 04 June 2014

Herdin Record #: NCR-OLFU-14043014260818

Determination of the cytotic and the preliminary assessment of the antimitotic activity of the crude extract of Antigonon leptopus (Cadena de amor) flower.


1Kristine N. Concepcion Principal Investigator
2Robelyn B. Lorca Researcher
3Joana Nica D. Nadurarta Researcher
4Rezyl P. Tuba-on Researcher
5Angelita A. Rodriguez Adviser

Publication Information

Publication Type:
Thesis Degree:
Publication Date:
March 2014
Our Lady of Fatima University


The study focuses on on determining the cytotoxic and antimitotic activity of Antigonon leptopus (Cadena de Amor) flower.


This study was conducted to assess the cytotoxic and preliminary assessment of the antimitotic activity of Antigonon leptopus flower extract. The first step in the identification of the cytotoxic and antimitotic activity of the Antigonon leptopus flower is the screening of the plant extract's active chemical constituents. The plant sample was air dried, macerated and extracted to be able to detect the constituents present. Based on the results we obtained, Antigonon leptopus contains triterpenoids, flavonoids and tannins, which are the manifestations for the cytotoxic and antimitotic activity.

Cytotoxic activity of Antigonon leptopus flower extract was tested using Brine shrimp lethality assay. It was evaluated at three different concentrations i.e. 10, 100 and1000 ppm and potassium dichromate was used as the reference standard. In the experimentation, the number of surviving nauplii was counted. The cytotoxic effect of Antigonon leptopus flower extract at 1000 ppm was relatively better than that of the other two test concentrations (10 and 100 ppm). All of the three concentrations of Antigonon leptopus flower extract screened for activity against A. salina larvae exhibited cytotoxic activity. The Antigonon leptopus flower extract had 50% lethal concentration (LC50) value of less than 1000 ppm (232.26 ppm at 24 h), confirming that the extract was cytotoxic.

On the other hand, the preliminary assessment of the antimitotic activity of Antigonon leptopus flower extract was tested using Allium cepa test. The root tips of A. cepa were treated with different concentrations (100 ppm and 1000 ppm) of crude extract for 96 h along with negative control. The length of roots was measured afterwards. The researchers observed that the length of onion roots

placed on the different concentrations of A. leptopus crude extract were significantly shorter compared to the onion roots placed on distilled water only. This may indicate that A. leptopus crude extract exhibited an inhibition of the growth of the onion roots.

Physical Location

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1. Aulton, M. E.. Aulton’s Pharmaceutics: The Design and Manufacture of Medicines (3rd Edition). Hungary: Harcourt Publishers, 2007.
2. Bauer, L. A.. Clinical Pharmaceutics Handbook Maryland: McGraw-Hill Companies, 2006.
3. Blouin, R. A., DiPiro, J. T., Pruener, J. M., Spruill, W. J., Wade, W. E.. Concepts in Clinical Pharmacokinetics (5th Edition). Maryland: American Health-System Pharmacists, Inc, 2010.
4. Budarari, S. . The Merck Index (12th Edition) Whitehouse Station, NJ: Merck & Co., , 1996.
5. DiGangi, F. , Knevel, A. . Jenkins’ Quantitative Pharmaceutical Chemistry (7th Edition). United States of America: McGraw-Hill, Inc. , 1977.
6. Evans, W. C.. Trease and Evans Pharmacology (15th Edition). Edinburgh: WB Saunders Company, 2002.
7. Guevara, B. . A Guidebook to Plant Screening: Phytochemical and Biological Manila, Philippines: UST Publishing House, 2005. 24-52.
8. Holdford, D. A.. Introduction to Hospital & Health-System Pharmacy Practice (1st Edition). USA: Bethesda, MD: American Society of Health-System Pharmacist, 2010.
9. Nathan, A. . Nonprescription Medicines (4th Edition) London: Pharmaceutical Press. , 2010.
10. Langley, C. A., Belcher, D. . Pharmaceutical Compounding & Dispensing (2nd Edition). London: Pharmaceutical Press, 2012.
11. Malone, P. M., Kier, K. L., Stanwich, J. E.. Drug Information Guide (International Edition) USA: McGrawHill Companies Inc, 2012.
12. Ogden, S. J.. Calculation of Drug Dosage (5th Edition). USA: Meslay Yearbook, Inc, 1995.
13. Pankaja, N. . Essentials of Biochemistry (1st Edition). Jaypee Brothers Medical Publishers, 2012.
14. Posey, M. . Pharmacy an Introduction to the Profession (2nd Edition) American Pharmacist Association, 2009.
15. Remington, J. . The Science of Practice of Pharmacy (21st Edition). Philadelphia: Lippincott. , 2006.
16. Mutschler, E. , Dorendorf , . Drug Actions: Basic Principles & Therapeutics Aspects (1st English Edition). Stultgerd, Germany: MedPharm Scientific Pub, 1995.
17. Pagliaro, L. A., Pagliaro, A. M.. Pagliaro’s Comprehensive Guide to Drugs &Substances of Abuse (2nd Edition). Washington DC: American Pharmacists Association, 2009.
18. Pray, W. S.. Nonprescription Product Therapeutic (2nd Edition). 351 West Camden Street, Baltimore, Maryland, USA: Lippincott Williams & Wilkins, 2006.
19. Shah, B. , Seth, A. K.. Textbook of Pharmacognosy & Phytochemistry New Delhi: Elsevier, 2010.
20. Turner,, P. , Richens, A. . Clinical Pharmacology London: Churchill Livingstone, 1973.
21. Woodtow, R. . Essentials of Pharmacology for Health Occupations (5th Edition). 15 Shenton Way #01-01 UIC Building Singapore: Thompson Learning Asia, 2007.
22. Abu-Rabia , A. . "Urinary diseases and ethno botany among pastoral nomads in the Middle East" Journal of Ethnobiology and Ethnomedicine 4(1): 2005 - [External Link]
23. Allan, L. A., Clarke, P. R.. "Phosphorylation of caspase-9 by CDK1/cyclin B1 protects mitotic cells against apoptosis. " Molecular Cell 26, 301-310, 2007
24. Al-Qura, S. . "Ethnobotanical survey of folk toxic plants in southern part of Jordan" Toxicon 46, 119-126, 2005
25. Andrade , L. F., Campos , J.M. S., Davide, L. C.. "Cytogenetic alterations induced by SPL (spent potliners) in meristematic cells of plant bioassays," Ecotoxicol. Environ. Saf., 71, 706-710, 2008
26. Chen, J. G., Horwitz, S. B.. "Differential mitotic responses to microtubulestabilizing and -destabilizing drugs" Cancer Res 62, 1935-1938, 2002
27. Cheryl , A. L.. "Ethno medicines used in Trinidad and Tobago for urinary problems and diabetes mellitus of Ethno biology and Ethno medicine" Journal of Ethnobiology and Etyhnomedicine 2, 45, 2006
28. Chistokhodova , N. , Nguyen , C. , Calvino, T. , Kachirskaia, I. , Cunningham, , Miles, D. H.. "Antithrombin activity of Medicinal plants from Central Florida" J.Ethnopharmacol 81, 277-280, 2002
29. Ngelangel, Corazon A.. "Cancer and the Philippine Cancer Control " Japanese Journal of Clinical Oncology 32, 552-561, 2002
30. Cushnie , L. , Lamb, A. J.. "Antimicrobial activity of flavonoids.Int J AntimicrobAgents" PubMed 26(5): 343-356, 2005
31. De Sousa , R. R., Queiroz , K. C., Souza, A. C., Augusto , A. C., Miranda , M. A.. "Phosphoprotein levels, MAPK activities and NFkappaB expression are affected by fisetin" J Enzyme Inhib Med Chem 22(4): 439-444, 2007
32. Farnsworth, N. R., Akerele, O. , Bingel, A. S., Soejarto, D. D., Guo, Z. . "Medicinal plants in therapy. " Bull. World Health Organ 63(6): 965-981, 1985
33. Fiskesjo, G. . "The Allium test: An alternative in environmental studies, the relative toxicity of metal ions." Mutat Res 197, 243-260, 1988
34. Gay, L. , Mills, G. , Airasian, P. . Educational research: Competencies for analysis andapplications. New Jersey: Pearson Education, Inc., 2006.
35. Gazzaneo , L. R., Paiva de Lucena , R. F., Paulino de Albuquerq, U. . (2005). Knowledge and use of Medicinal plants by local specialists in an region of Atlantic Forest in the state of Pernambuco . Available from: http://http.// - [External Link]
36. Hanazaki , N. , Tamashiro, J. Y., Leitao-Filho , H. , Gegossi , A. . Diversity of plant uses in two Caicaras communities from the Atlantic forest coast Brazil: Biodiversity and Conservation , 2000. 597-615.
37. Havsteen, B. H.. "The biochemistry and medical significance of flavonoids" Pharmacology & Therapeutics; 96, 67-202, 2002
38. Havey, M. J., Rabinowitch, H. D., Currah, L. . Genome organization in Allium. In Allium Crop Science, (Eds.) United Kingdom: Recent Advances, CABI Publishing, 2002. 59-79.
39. Gascoigne, Karen E., Taylor, Stephen S.. "How do anti-mitotic drugs kill cancer cells? " Journal of Cell Science 122, 2579-2585, 2009
40. Jian, Wu . "Manufacture of traditional Chinese medicine with Anti viral, Anti inflammatory and detoxicating effects." Faming ZhuanliShenqingGongkaiShuomingshu 1(840): 147, 2006
41. Jordan, M. A., Wilson, L. . "Microtubules as a target for anticancer drugs" Nat. Rev. Cancer 4, 253-265, 2004
42. Jordan, M. A., Thrower,, D. , Wilson, L. . "Mechanism of inhibition of cell proliferation by Vinca alkaloids" Cancer Res 51, 816-825, 1991
43. Jordan, M. A., Wendell, K. , Gardiner, S. , Derry, W. B., Copp, H. , Wilson, L. . "Mitotic block induced in HeLa cells by low concentrations of paclitaxel (Taxol) results in abnormal mitotic exit and apoptotic cell death" Cancer Res 56, 816-825, 1996
44. "Cytotoxicity Evaluation of Jordanian Wild Plants using Brine Shrimp Lethality Test" Faculty of Pharmacy, Applied Science University 8(1): 12-17, 2004
45. Nowakowska, J. , Kura, M. , S´ liwin´ ska , E. , Pilarski , R. , Ilasz, R. , Tykarska, T. , Zobel, A. , Gulewicz, K. . "Changes in chromosome structure, Mitotic activity and nuclear DNA content from cells of Allium test induced by bark water extract of Uncariatomentosa(Willd.) DC" J. Ethnopharmacol 107, 211-221, 2006
46. Latha, P. G., Panikkar , K. R.. "Chemoprotective effect of IxoracoccineaL. flowers on cisplatin induced toxicity in mice, " Cancer Lett 130, 197-202, 1998
47. Lehninger , L. A.. Nelson. Principles of biochemistry, 1st edition. .Delhi: CBS Publishers and Distributors, , 1993. 725.
48. Leme , D. M., Marin-Morales , M. A.. "Chromosome aberration and micronucleus frequencies in Allium cepacells exposed petroleum polluted water – a case study" Mutat. Res., 650, 80-86, 2008
49. (2005). Ethno-diversity within current ethnopharmacology as part of Israeli traditional medicine . Available from: - [External Link]
50. Mangale , S. M., Chonde , S. G., Raut , P. D.. "Use of Moringaoleifera (drumstick) seed as natural absorbent and an antimicrobial agent for ground water treatment" Res.J.Recent Sci 1(3): 31-40, 2012
51. McLaughlin, J. L., Rogers, L. L.. "The use of biologicalassays to evaluate botanicals" Drug Information Journal 32, 513-524, 1998
52. Meyer, B. N., Ferrigni, N. R., Putnam, J. E., Jacobsen, L. B., McLaughlin, J. L.. "Brine shrimp: a convenient general bioassay for active plantconstituents" PlantaMedica 54, 31, 1982
53. Mitchell, S. A., Ahmad, M. H.. "A review of medicinal plant research at the University of the West Indies, Jamaica" West Indian Medicinal Journal 55(4): 243-253, 2006
54. Mondal , D. , Mondal, T. . "A review on efficacy of Azadirachtaindica A. juss based biopesticides: an Indian perspective" Res.J.Recent Sci 1(3): 94-99, 2012
55. Mukherjee, P. K.. "Quality control of herbal drugs: An approach to evaluation of botanicals; Business Horizon" New Delhi 2, 2002
56. Niikura, Y. , Dixit, A. , Scott, R. , Perkins, G. , Kitagawa, K. . "BUB1 mediation of caspase-independent mitotic death determines cell fate. " J. Cell Biol 178, 283-296, 2007
57. Nogales, E. . "Structural insights into microtubule function" Annu. Rev Biochem. 69, 277-302, 2000
58. Panvichian, R. , Orth, K. , Day, K. C., Pilat, M. J., Pienta, K. J.. "Paclitaxel-associated multimininucleation is permitted by the inhibition of caspase activation: a potential early step in drug resistance." Cancer Res. 58, 4667-4672, 1998
59. Pei, S. J.. "Ethno botanical approaches of traditional medicine studies: Some experiences from Asia.Pharmaceutical Biology " Journal of Ethnobiology and ethnomedicine 39, 74-79, 2001
60. (2011). Practical Biology . Investigating mitosis in allium root tip squash. Available from: - [External Link]
61. Rai, S. S., Wolf, J. . "Localization of the vinblastine-binding site on beta-tubulin" J. Biol. Chem 271, 14707-1471, 1996
62. Onion Root Mitosis. Allium Root Tip. Available from: - [External Link]
63. Rossato, S. C., Leitao-Filho , H. , Gegossi , A. . "Ethnobotany of Caicaras of the Atlantic forest coast (Brazil)" Economic botany 53, 387-395, 1999
64. Satoskar, R. S., Bhandarkar, S. D.. Pharmacology and pharmacotherapeutics, 2nd edition Bombay: Popular Prakashnan Publishers, 1989. 704.
65. "Science and Plants for School " Staining a root tip and calculating its mitotic index: Student Guide. 1-4, 2012
66. Shachi, S. . "Antimitotic activity of a New Compound Isolated from the Flower ofProsopisjuliflora. " Research Journal of Recent Sciences 1(16): 22-26, 2012
67. William , Carey M., VenkatRao, N. , Ravikumar , B. , Krishna, Mohan G.. "Preliminary studies of analgesic and anti-inflammatory properties of Antigononleptopus Hook. &Arn, roots in experimental models." J. Health Science 54(3): 281-286, 2008
68. Xu, R. , Zhao, W. , Shao, B. , Qin , G. . "Studies on bioactive saponins from Chinese medicinal plants. " AdvExp Med &Bio 404, 371-382, 1996
69. (2011). Coral Vine. Flowers of India.. ©1996-2011 LC Tallahassee, Florida USA. Available from: - [External Link]