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Submitted: 22 October 2012 Modified: 22 October 2012
HERDIN Record #: R04A-DLSHSI-12102209212838

Bacteremia in the Medical Intensive Care Unit of De La Salle University Medical Center.

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Objectives: Identify rates of true and false positive blood cultures, specify possible sources of contamination and determine their clinico-demographic correlations.
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Design: Prospective observational study.
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Patents/ Subject selection: All adult patients admitted in the MICU of DLSUMC who reported to have blood cultures from January to June 2008.
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Outcome Measures: Patients were assessed for clinical evidences of bacteremia. Blood culture isolates were classified into true positive or false positive results. Isolation and contamination rates of positive blood cultures were then identified. The clinico-demographic data was correlated to the true/false positive blood culture using non-parametric analysis.
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Results: 166 blood cultures were obtained from 90 patients and 38 or 22.89% were positive blood cultures, out of this, 30 or 18% were considered true pathogen and 8 or 4.82% as contaminants. Blood cultures collected by medical technologists had a significant lower rate of contamination than those taken by the nursing staff with p-value of 0.032. The presence of fever, hypotension, leukocytosis, antibiotic use, accompanying co-morbidities and immunocompromised state showed no significant correlation with true bacteremia.
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Conclusion: Prevention of blood culture contamination highly depends on scrupulous aseptic techniques, sufficient amount of blood and on a well-trained and dedicated phlebotomist

Objectives

Identify rates of true and false positive blood cultures, specify possible sources of contamination and determine their clinico-demographic correlations.

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