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Submitted: 10 September 2012 Modified: 29 May 2013
HERDIN Record #: NCR-RITM-12091009012696

Isolation and partial characterization of dengue virus type 2 and 4 strains from dengue fever and dengue haemorrhagic fever patients from Mindanao, Republic of the Philippines.


Objective: Isolation of dengue virus from dengue fever and dengue hemorrhagic fever cases from Mindanao, Republic of the Philippines.

Methods: 12 patients with clinically suspected dengue fever (DF) or dengue hemorrhagic fever (DHF) presenting in four regional hospitals between August adn September 1995 on Mindanao were enrolled in the study. Dengue virus was isolated by inoculation of Ver/E6 or C6/36 cells with patient serum/ IgM antibodies were measured using a commercial test system. Up to 454 bp of the capsid region and 240 bp of the E/NS1 gene junction of different viral isolates were sequenced and phylogenetically analyzed.

Results: Virus could be isolated from seven patients, five isolates were typed as dengue virus type 2 and two as dengue virus type 4 by immunostaining with monoclonal antibodies or by RT/PCR. Phylogenetic analysis confirmed a close relationship of the dengue virus type 2 isolates with viruses isolated in the Philippines in 1983 and 1988.

Conclusion: As observed in studies from other parts of South East Asia, dengue virus type 2 was readily isolated from dengue hemorrhagic fever cases. Dengue virus type 2 and 4 circulate in Mindanao, Philippines, with dengue type 2 being responsible for most of our severe DF or DHF cases

Publication Type
Journal
Publication Sub Type
Journal Article, Original
Title
Tropical Medicine and International Health
Frequency
Monthly
Publication Date
May 2000
Volume
5
Issue
5
Page(s)
325-329

Objectives

Isolation of dengue virus from dengue fever and dengue hemorrhagic fever cases from Mindanao, Republic of the Philippines.

LocationLocation CodeAvailable FormatAvailability
RITM Fulltext Print Format
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