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Submitted: 02 August 2012 Modified: 29 May 2013
HERDIN Record #: NCR-RITM-12080216040023

Increased phagocytosis of platelets from patients with secondary dengue virus infection by human macrophages.

1Shoko Honda,
2Mariko Saito,
3Efren M. Dimaano,
4Philip A. Morales,
5Maria T. Alonzo,
6Lady-Anne C. Suarez,
7Natsuki Koike,
8Shingo Inoue,
9Atsushi Kumatori,
10Ronald R. Matias,
11Filipinas F. Natividad,
12Kazunori Oishi
Department of Clinical Medicine and Virology - Institute of tropical Medicine Nagasaki University,
Department of Virology/Graduate School of Medicine - Tohoku University,
Department of Disaster Prevention System/Faculty of Risk and Crisis Management - Chiba Institute of Science,
Laboratory for Clinical Research on Infectious Diseases - Osaka University,
International Research Center for Infectious Diseases - Osaka University,
Research Institute for Microbial Diseases - Osaka University,
Department of Blood Borne Diseases - San Lazaro Hospital,
Research and Biotechnology Division - St. Luke's Medical Center


The relationship between the percent phagocytosis of platelets by differentiated THP-1 cells was examined using flow cytometry and the peripheral platelet counts, as well as platelet-associated IgG (PAIgG) in 36 patients with secondary dengue virus (DV) infections.The percent phagocytosis and the levels of PAIgG were significantly increased in these patients during the acute phase compared with the healthy volunteers. The increased percent phagocytosis and PAIgG found during the acute phase significantly decreased during the covalescent phase. An inverse correlation between platelet count and the percent phagocytosis (P=0.011) and the levels of PAIgG (P=0.041) was found among these patients during the acute phase. No correlation was found, however, between the percent phagocytosis and the levels of PAIgG. Our present data suggest that accelerated platelet phagocytosis occurs during the acute phase of secondary DV infections, and it is one of the mechanisms of thrombocytopenia in this disease

Publication Type:
Publication Sub Type:
Journal Article, Original
St. Luke's Journal of Medicine
Publication Date:
January-June 2010
St. Luke's Medical Center

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