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Submitted: 16 August 2011 Modified: 27 February 2018
HERDIN Record #: PCHRD08161102082028

A preliminary investigation on the anti-microbial properties of "Biab".

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Aqueous and alcoholic extracts of the tuber were tested on a number of microorganisms namely: Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27813, Staphylococcus aureus ATCC 25923, Staphylococcus aureus UPCC 234, Streptococcus viridans UPCPH, Candida albicans UPCC 2129, Tricophyton mentagrophytes UPCPH, T. mentagrophytes UPCC. Except for the two strains of T. mentagrophytes all the test organisms were subjected to the Kirby-Bauer Disk Diffusion Method. Results showed that the alcoholic extract prepared by soaking grated tuber in the solvent on a 1:1 (w/v) ratio, caused a moderate inhibitory effect on S. aureus ATCC but elicited a weak response from Streptococcus viridans. All the rest showed a negative response to the extract. The aqueous extract likewise resulted in a weak inhibitory action to both S. aureus ATCC and S. viridans, and likewise got a negative response from all the rest. Mature and young tubers did not differ in their effects on the test organisms.The minimum inhibitory concentration (MIC) of alcoholic and water extract was also determined for S. aureus ATCC and S. viridans. The MIC for the alcoholic extract was observed to be on the 6th dilution tube for both organisms. This represented 1.56% crude extract. The aqueous extract showed MIC on the 4th dilution tube containing 6.25% of the original extract. This again shows that the alcoholic extract is more effective in inhibiting the growth of the two microorganisms. When alcoholic and aqueous extracts were tested on the two strains of T. mentagrophytes the amount of growth exhibited by the filamentous fungus was compared. Different amounts of the crude extracts were added to make up 10 ml Sabouraud Agar medium per plate. Alcoholic extracts of 1.0 ml, 0.5 ml, and 0.4 ml proved effective in preventing growth for both strains. A similar response was observed in aqueous extracts of 1.0 ml and 0.5 ml. A corresponding increase in mycelial growth was observed for both strains with decreasing concentrations of the two extracts in the medium. However, a 10 ml alcoholic extract and a 0.2 ml aqueous extract produced growths resembling their respective controls. The results suggest that alcoholic extract is more effective than aqueous extract in controlling the growth of S. aureus ATCC, S. viridans, and T. mentagrophytes. This reaction can be due to a synergistic interaction between alcohol and the active component of the plant or that the active principle is much more soluble in alcohol and hence a higher concentration is present in alcoholic extracts. (Author)

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