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Submitted: 15 August 2011 Modified: 05 May 2017
HERDIN Record #: PCHRD08151102083617

Cloning of hepatitis B virus (HBV) deoxyribonucleic acid (DNA) and development of an HBV DNA probe.

1Rhodora G. Zafra
Department of Biochemistry and Molecular Biology, College of Medicine, University of the Philippines, CM-UPM,

Abstract

The hepatitis B virus (HVB) deoxyribonucleic acid (DNA) was isolated from serum and cloned in the plasmid. The recombinant plasmid was then purified and later labeled with a non-radioactive nucleotide analog. An assay for detecting labeled recombinant plasmid DNA immobilized on to solid support was tested.THe next step involves the use of labeled recombinant plasmid DNA as prove for the detection of HBV DNA in serum samples.

1.
Publication Type:
Research Project
Date:
June 30, 1991-May 31, 1992

LocationCall NumberAvailable FormatAvailability
Philippine Council for Health Research and Development Library Abstract Print Format (Request Document)