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Submitted: 15 August 2011 Modified: 12 January 2017
HERDIN Record #: PCHRD08151101082385

An in vivo study on the effects of Apium graveolens Linneaus leaf extract on serum uric acid levels of potassium-oxonate induced hyperuricemic albino rats.

1Roderick Dizon,
2Maria Therese Dela Rosa,
3Rodney De Guzman,
4Ma. Isabel Cristi,
5Maj Maj Deriada,
6Donna De Leon,
7Joseph Cuebillas,
8Jonel Constantino,
9Joseph Diego,
10Noemi  Del Rosario,
11Maricel De Guzman,
12Aline Cortez,
13 Christopher Delos Reyes,
14Michael De Jesus,
15Catherine Cua,
16Tarhata Dilabakun,
17Abraham De Vera,
18Eduardo Dayacap,
19Mary Joy Coronel
Department of Pharmacology, University of the East Ramon Magsaysay Memorial Medical Center, UERMMMC, Department of Preventive and Community Medicine, University of the East Ramon Magsaysay Memorial Medical Center, UERMMMC,


Preliminary studies, conducted to determine the lethal dose, placed the LD 50 at 13 g/kg. body weight. Hyperuricemia was induced in 40 randomly selected rats by gastric gavage feeding using 5% Potassium oxonate and 1% uric acid. The hyperuricemic rats were randomly divided into 4 groups: a positive control group which was givel allopurinol, a negative control group A (low dose or 3.25 g/kg body weight or 1/4 LD50) and kintsay group B (hig dose or 6.5 g/kg BW or 1/2 LD50). Uric acid levels were undertaken before and after induction of hyperuricemia to veryfy if the serum uric acid levels did increase and 24 hours after administration of the allopurinol. A. graveolens and distilled water to verify the decrease of uric acid levels due to drug action. Determination of serum uric acid levels was done through the phosphotungstate method (FAME). It was observed that allopurinol exhibited the greatest decrease in serum uric acid levels (0.86 mg/dL), followed by Apium graveolens at 6.5 mg/dL (0.39 mg/dL), A. graveolens at 3.25 g/dL (0.31 mg/dL), and lastly by water (0.08 mg/dL). Using hypothesis testing of means, it could not be included, however, that, at 0.05 level of significance, A. graveolens L. is significantly different from water. At 6.5 g/kg, it could not be proven either that A. graveolens was significantly less effective than allopurinol. At. 3.25 g/kg, A. graveolens was significantly less effective than Allopurinol. It is recommended that a larger amount of subjects and sequential determination of serum uric acid levels be performed to verify if Apium graveolens Linn. could really lower uric acid levels.

Publication Type:
Research Project


To determine the effect of Apium graveolens Linn. (kintsay)on serum uric acid levels of hyperuriccemic albino rats.

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