Report an error   

Submitted: 16 May 2007 Modified: 13 February 2014
HERDIN Record #: PCHRDPC880015

Investigation of some Philippine plants for antimicrobial substances.

1Edna  V. Ramos,
2Roseanna M. De La Cruz,
3Victoria A. Masilungan,
4Flora B. Beloy


The antimicrobial activity of 848 extracts prepared from 106 species of Philippine plants belonging to 44 families was determined by the disc agar diffusion method of assay. Eight solvents (ether, petroleum ether, acetone, alcohol, benzene, chloroform, water and 1 per cent sodium bicarbonate) were used to extract substances from each plant
Of the plants tested, 62 or 58 per cent were shown to contain substances inhibitory to Bacillus subtilis, Micrococcus aureus, Escherichia coli and Mycobacterium tuberculosis 607. The highest incidence of antimicrobial activity was obtained with alcohol extractions followed by ether, acetone, water, benzene, chloroform, 1 per cent sodium bicarbonate and petroleum ether in this order. The 9 most active plants which demonstrated exceptionally high values of antimicrobial activity are :Alphonsea arborea, Alstonia scholaris, Artocarpus blancoi, Caesalpinia crista, Callicarpa formosana, Diplodiscus paniculatus, Pisonia umberllifera, Talauma villariana and Zingiber officinale.

The occurence of antimicrobial principles in some plants of India, Canada, Australia, America, Japan and Taiwan have been reported as early as man's search for natural sources of remedial cures for diseases. A number of Philippine plants were also screened for the presence of substances inhibitory to Micrococcus aureus and Escherichia coli by Masilungan et. al. employing alcohol and water for extractions.

The study presented here is an extension of in-vitro studies with the inclusion of more test organisms and more solvents for extracting active substances. This report deals

Publication Type:
Publication Sub Type:
Journal Article, Original
Philippine Journal of Science
Publication Date:
December 1976
Department of Science and Technology

LocationCall NumberAvailable FormatAvailability
Philippine Council for Health Research and Development Library Box No. 119/LA Fulltext pdf (Request Document)
1. Andrews, D H, Borchardt, K A. "." Antib. and Chemo 11(5): 340, 1961
2. Trease, G E. "A textbook of pharmacognosy 9th ed" Bailliere, Tindall and Caselle , 821, 1966
3. Sequin, L , Kutchan, A H. "." Antib and Chemo 11(5): 340, 1961
4. Singer, D E, Pierce, L V, Yawn, E C. "." Tex. Rep. Biol. and Med. 11(1): 122, 1953
5. Randall, W A, Grove, D C. "Assay methods of antibiotics: a laboratory manual" Medical Enzyclopedia , 238, 1960
6. Khaleque, K A, Muazzam, M G. "." The Journal of Tropical Medicine and Hygiene 63(3): 70, 1960
7. de Leon, P , Maranon, J , Valencia, V V, Diokno, N C, Masilungan, V A. "." Philippine Journal of Science 84(3): 275, 1955
8. McClung, L S, Wax, P W, Sanders, D W. "." Journal of Bacteriology (49): 611, 1945
9. Tanabe, S . "." Japanese Journal of Bacteriology (9): 475, 1954
10. Valencia, V V, Maranon, J , Masilungan, V A. "." Journal of Philippine Pharmaceutical Association 5(42): 72, 1955
11. Koo, W H. "Preliminary screening test for antimicrobial activity on Taiwan fresh plants" Proceedings of International Symposium Medicinal Plants , 155, 1966
12. Taylor, A , McKenna, G F, Gibson, S M, Burlarge, H M. "." Tex. Rep. Biol. and Med (12): 229, 1954
13. Bishop, C J, Macdonald, R E. "." Canadian Journal of Botany 29(3): 260, 1951
14. Brice, H E, Atkinson, N . "." Australian Journal of Experimental Biological Medical Science (33): 547, 1955
15. Datta, S C. "Production of medicinal plants in India" Proceedings of International Symposium on Medicinal Plants , 57, 1966
16. Venkataraman, P R, Pandalai, K M, George, M . "." Journal of Science & Indian Research , 42, 1947
17. Venkataraman, P R, Srinivasa, R S, Raghunandana, R R. "." Journal of Science & Indian Research , 31, 1946
18. Wickerman, K , Tunevall, G , Ericson, H M. "." Scandinavian Journal of Clinical Laboratory Investigations (12): 408, 1960
19. Beloy, F B. "." Philippine Journal of Science 1(13): 4, 1967