Report an error   

Submitted: 16 May 2007 Modified: 04 December 2013
HERDIN Record #: PCHRDPC980868

Application of radioimmunoassay methods for malaria detection in two selected endemic areas in the Philippines.

2Erlinda S. Natera ,
3Cielo J. Pasay,
4 Tiu WU


Radioimmunoassay (RIA) technique was used with the synthetic peptide, (NANP) 3 in detecting anti-sporozoite antibody (against Plasmodium falciparum) in serum of persons residing in two (2) endemic areas in the Philippines. Entomological surveys for sporozoite detection in mosquito vectors utilizing monoclonal antibodies (2A10 for P. falciparum and 2F2 for P. vivax) were likewise conducted in the same areas where serological surveys were performed. These two areas are located on separate islands, with varying malaria transmission seasons and levels of endemicity
Initial findings showed positive response to thh CSP antigen (NANP)3 in detecting anti - P. falciparum antibodies in sera. Infection with sporozoites of P. falciparum and P. vivax in mosquito vectors were detected using monoclonal antibodies 2A10 and 2F2 respectively. The latter procedure was shown to be more sensitive than dissection of mosquito salivary glands
Initial study shows a heightened level of anti-(NANP)3 antibodies in both populations prior to the generally accepted peak of malaria season indicating that RIA with CSP antigen and specific MAbs can be a useful epidemiological tool for understanding the dynamics of malaria transmission as well as in monitoring control programmes based on reducing manvector contact.

Publication Type:
Publication Sub Type:
Journal Article, Original
Philipp Tech J
Publication Date:
July-September 1995
Department of Science and Technology-Science and Technology Information Institute

LocationCall NumberAvailable FormatAvailability
Philippine Council for Health Research and Development Library Box No. 12 Fulltext Print Format (Request Document)
1. Salazar, Nelia P, Gomez, Mleba . "Varying malaria epidemiological patterns in the Phil. Implications for control. Paper presented at 25th annual general meeting and seminar at Malaysian Society of Parasitology and Tropical Medicine, Institute for Malaria Research Kuala Lumpur Malaysia, 24" , 1989
2. Nardin, EH , Nussenweig, RS . "Circumsporozoite proteins of human malaria parasites plasmodium falciparum and plasmodium vivax" J Exp Med 156(20): 1992
3. Dame, J , Williams, JL , McCutcham, TF . "Structure of the gene encoding the immunodominant surface antigen on the sporozoiter of the human malaria parasite plasmodium faciparum" Sciene 225, 593-599, 1984
4. Zavala, F . "Circumporozite protein of malaria parasite contain a single immunodominant legion with two or more identical epitopes" Journal of Experimental Medicine 157, 1947-1987, 1993
5. Zavala, F . "Synthetic peptides for detection of humoral immunity to plasmodium falciparum sporozoite" Journal of Immunological Methods 93, 55-61, 1988
6. Druilhe, P . "Levels of antibodies to plasmodium falciparum sporozoite surface antigens reflect malaria transmission and are persistent in the absence of reinfection" Infection and Immunity 53, 393-397, 1986
7. Kasemsuth, R . "Detection of malaria sporozoite by immunoraqdiometric assay, " Southeast Asian Journal of Tropical Medicine Public Health 19(1): 79-88, March 1988
8. Zavala, F . "Monoclonal antibodies to circumsporozoite protein identify the species of malaria parasite in infected mosquitoes" Nature 299, 737-738, October 1982
9. Collin, F . "Laboratory assessment of a specie-specific radio-immunoassay for the detection of malaria sporozoite in mosquitoes (Diptles Culicidae)" Journal of Medical Entomology 22(2): 121-129, March 1985
10. Marsh, K . "Anti-sporozoite antibodies and immunity to malaria in a rural Gambian population" Transaction of the Royal Society of Tropical Medicine and Hygiene 82, 532-537, 1988
11. Nardin, EH . "Antibodies to sporozoites: Their frequent occurrence in individual living in an area of hyper-endemic malaria" Science 208, 597-599,
12. Oberst, RB . "Immune response to the circumsporozoite protein of plasmodium falciparum in longitudinal study: Palawan Philippines. Unpublished Report: US Naval Medical Command, Bethesda, maryland" , 20814-5044, 1988
13. Salazar, NP . "The malaria situation in the Philippines, a critique. Paper presented at the Phil, Council for Health Research and Development (DOST) Congress, Phil Heart Center for Asia, Quezon City Philippines" , 1988
14. Pye, J , Quilici, D . "Chloramine T method of radio-labelling. Water and Eliza Hall Institute Melbourne, Australia" ,
15. Oliveira, J . "Use of immuno-radiometric assays employing monoclonal antibodies of synthetic peptides for the study of malarial transmission and immunity in migrant population of endemic areas in the amazon basin. A paper presented at the 1990 International Atomic Energ" , 1990