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Submitted: 16 May 2007 Modified: 19 December 2014
HERDIN Record #: PCHRDHE901100

Experimental infection of mosquitoes with malarial parasites.


The project was started in 1983 to help establish at RITM a research facility in medical entomology particularly to study vectorial and parasitological aspects of malaria. There was then a need for experimental tools for studying host-parasite-vector interrelationship, screening antimalarials, and testing vector control agents. The specific objectives were: 1) to establish laboratory-bred colonies of anopheline mosquitoes, 2) to determine their susceptibility to infection with malarial parasites, and 3) to assess the effect of drugs on the gametocytes and developmental stages in mosquitoes. These entailed site visits to endemic areas, collection of mosquitoes from natural habitat, insectary procedures, in vitro cultivation of plasmodia, patient recruitment, and multifarious laboratory tests. To date, seven technical papers have been produced and presented in scientific meetings. Three of these were published and four are in press. Accomplishments include: 1) successful colonization and infection of Anopheles litoralis with Plasmodium falciparum gametocytes, 2) demonstration of gametocytocidal activity of clindamycin, and 3) application of diagnostic techniques e.g. G-6-PD assays, immunoflourescent antibody test, and in vitro microtest for drug sensitivity. At present, laboratory-bred mosquitoes are used in test of neem (Azadirachta indica), a botanical insecticide, as a prospective vector agent. Experimentally infected mosquitoes serve as controls in immunoradiometric assays for malaria detection.

Publication Type
Research Project
Date
LocationLocation CodeAvailable FormatAvailability
Philippine Council for Health Research and Development Library RA 644.M2 S4 1989 Fulltext Print Format (Request Document)

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